The Role of Laminarin in improving Inflammatory, oxidative stress marker, and liver function against Aeromonas hydrophila toxicity

Abstract

Background: Aeromonas hydrophila, originally named Bacillus hydrophilus fuscus, was the first discovered species of Aeromonas. It is a gram-negative bacterium that is widespread in inland aquatic environments, benthic sediments, and other aquatic organisms. Although it is an emergent bacterial pathogen in humans and aquaculture, it is part of the normal intestinal microflora of healthy fish and is considered an opportunistic agent. The aim of the study: Effect of the fungal cell wall, Laminarin (b-1,3-D-glucans), on some biochemical constituents in the blood and liver of the albino rats (Rattus norvegicus) was determined. Material and Method: Rats were intraperitoneally injected with 0.2 ml of 1x10⁷ Aeromonas hydrophila suspended in 0.9% saline solution every day for eighteen days. Another group of rats was injected (i.p.) with 0.2 ml of the same bacterial concentration for six days and then injected with 0.2 ml of 15 mg/100 b.wt of Laminarin at different time intervals. Result: Daily observations of the animals after their injection with A. hydrophila showed gradual paleness of the eyes during the period of injection; normal dark red-colored eyes turned gradually to clearly pale red after 15 days. The estimation of liver function, inflammatory and oxidative stress markers in serum as well as liver homogenate showed increases in their levels during almost all time intervals after bacterial infection, while laminarin enhanced almost all these values. Conclusion: The administration of laminarin helps improve of Aeromonas hydrophila toxicity. In addition, the symptomatic changes recovered to approximately normal status at all different times.